4DYH
Crystal structure of glycosylated Lipase from Humicola lanuginosa at 2 Angstrom resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM14 |
| Synchrotron site | ESRF |
| Beamline | BM14 |
| Temperature [K] | 77 |
| Detector technology | CCD |
| Collection date | 2011-11-09 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.97 |
| Spacegroup name | P 61 |
| Unit cell lengths | 139.955, 139.955, 80.537 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 48.400 - 2.000 |
| R-factor | 0.21643 |
| Rwork | 0.213 |
| R-free | 0.28290 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1dt3 |
| RMSD bond length | 0.022 |
| RMSD bond angle | 1.983 |
| Data reduction software | AUTOMAR |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.400 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Number of reflections | 60824 | |
| <I/σ(I)> | 8.6 | 2.5 |
| Completeness [%] | 99.4 | 99.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298 | 0.1M HEPES, O.1M Nacl, 1.6M Ammonium sulphate, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
