4DYE

Crystal structure of an enolase (putative sugar isomerase, target efi-502095) from streptomyces coelicolor, no mg, ordered loop

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 31-ID
Synchrotron site	APS
Beamline	31-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2012-02-03
Detector	RAYONIX MX225HE
Wavelength(s)	0.9793
Spacegroup name	I 4 2 2
Unit cell lengths	132.670, 132.670, 111.250
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	26.665 - 1.600
R-factor	0.1577
Rwork	0.157
R-free	0.17490
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2oqh
RMSD bond length	0.006
RMSD bond angle	1.139
Data reduction software	MOSFLM
Data scaling software	SCALA (3.3.16)
Phasing software	PHENIX
Refinement software	PHENIX (1.7.3_928)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	93.812	26.665	1.690
High resolution limit [Å]	1.600	5.060	1.600
Rmerge	0.101	0.041	0.796
Total number of observations		28993	135708
Number of reflections	65168
<I/σ(I)>	15.6	14.1	1
Completeness [%]	99.9	98	100
Redundancy	14.5	13.3	14.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	sitting drop vapor diffuction	4	298	Protein (10 mM Hepes pH 7.5, 150 mM NaCl, 10% glycerol, 1 mM DTT, 5 mM MgCl); Reservoir (0.2M Amm Formate pH 6.6, 20% Peg3350); Cryoprotection (Reservoir, + 20% ethylene glycol and 50 mM MgCl), sitting drop vapor diffuction, temperature 298K