4DYC
Crystal Structure of the terminase small subunit gp1 with D19R mutation of the bacterial virus sf6
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL7-1 |
| Synchrotron site | SSRL |
| Beamline | BL7-1 |
| Temperature [K] | 298 |
| Detector technology | PIXEL |
| Collection date | 2010-03-28 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 0.979500 |
| Spacegroup name | P 4 21 2 |
| Unit cell lengths | 88.570, 88.570, 73.550 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.805 - 1.800 |
| R-factor | 0.2084 |
| Rwork | 0.207 |
| R-free | 0.23470 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3hef |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.948 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((phenix.refine)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.850 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.042 | 0.384 |
| Number of reflections | 27608 | |
| <I/σ(I)> | 30.61 | 4.45 |
| Completeness [%] | 99.6 | 97.1 |
| Redundancy | 13.03 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 6.4 | 293 | 1.2M NAH2PO4/0.8M K2HPO4, 0.2M LI2SO4, pH 6.4, VAPOR DIFFUSION, temperature 293K |
