4DY3

crystal structure of Escherichia coli PliG, a periplasmic lysozyme inhibitor of g-type lysozyme

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2010-04-08
Detector	MAR scanner 345 mm plate
Wavelength(s)	1.54
Spacegroup name	P 41
Unit cell lengths	83.543, 83.543, 31.163
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.690 - 1.800
R-factor	0.1679
Rwork	0.166
R-free	0.21350
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	0.960
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.7.1_743))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	19.690	1.900
High resolution limit [Å]	1.800	1.800
Number of reflections	22521
<I/σ(I)>		3.8
Completeness [%]	93.0	91.1
Redundancy		4.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5	293	0.1M sodium acetate pH5.0, 0.15M NaCl, 25% w/v PEG6000, VAPOR DIFFUSION, HANGING DROP, temperature 293K