4DXW
Crystal structure of NavRh, a voltage-gated sodium channel
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-07-15 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.0050 |
| Spacegroup name | P 42 |
| Unit cell lengths | 163.437, 163.437, 61.071 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 45.329 - 3.052 |
| R-factor | 0.2389 |
| Rwork | 0.237 |
| R-free | 0.26800 |
| Structure solution method | SAD |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.452 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SHELXS |
| Refinement software | PHENIX ((phenix.refine: 1.7.3_928)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.160 |
| High resolution limit [Å] | 3.050 | 3.050 |
| Number of reflections | 31031 | |
| Completeness [%] | 99.7 | 99.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 291 | 5% PEG 8000 (v/v), 100mM HEPES-NaOH, pH 7.0, 100mM CaCl2, 10% Glycerol, 20% 1,4-Butandiol, VAPOR DIFFUSION, HANGING DROP, temperature 291KK |
