4DXR
Human SUN2-KASH1 complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-E |
| Synchrotron site | APS |
| Beamline | 24-ID-E |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-08-20 |
| Detector | ADSC QUANTUM 315 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 79.641, 79.641, 256.409 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 46.950 - 2.320 |
| R-factor | 0.177 |
| Rwork | 0.171 |
| R-free | 0.22700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4dxt |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.916 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: dev_975)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 85.500 | 2.400 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.076 | 0.707 |
| Number of reflections | 13944 | |
| <I/σ(I)> | 24.9 | 1.5 |
| Completeness [%] | 99.6 | 97.7 |
| Redundancy | 3.5 | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 291 | 0.1 M HEPES, PH 7.5, 0.2 M ammonium acetate, 0.30% DM, 25% 2-propanol, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
