4DXI
Crystal Structure of an Ancestor of All Faviina Proteins
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2008-02-01 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.5418 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 106.488, 93.058, 88.998 |
| Unit cell angles | 90.00, 92.72, 90.00 |
Refinement procedure
| Resolution | 28.120 - 1.600 |
| R-factor | 0.17484 |
| Rwork | 0.173 |
| R-free | 0.20981 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.402 |
| Data reduction software | d*TREK |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0110) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 28.350 |
| High resolution limit [Å] | 1.600 |
| Rmerge | 0.043 |
| Number of reflections | 112134 |
| <I/σ(I)> | 12.9 |
| Completeness [%] | 98.4 |
| Redundancy | 2.45 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.7 | 277 | 0.06M Tris, 0.1M magnesium sulfate, 20% PEG 4000, pH 7.7, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
