4DXG
Crystal structure of Staphylococcal Superantigen-Like protein 4 complexed with sialyl Lewis X
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2010-08-20 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.5418 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 61.880, 78.880, 97.701 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 24.340 - 2.500 |
| R-factor | 0.18792 |
| Rwork | 0.185 |
| R-free | 0.25433 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4dxf |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.779 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 24.420 | 2.640 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.169 | 0.747 |
| Number of reflections | 8563 | |
| <I/σ(I)> | 12 | 3.1 |
| Completeness [%] | 99.9 | 100 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.7 | 291.15 | 28% MPEG 5000, 0.18M PIPES, pH 6.7, VAPOR DIFFUSION, HANGING DROP, temperature 291.15K |
