4DW0

Crystal structure of the ATP-gated P2X4 ion channel in the closed, apo state at 2.9 Angstroms

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	CCD
Collection date	2011-03-10
Detector	ADSC QUANTUM 315
Wavelength(s)	0.97918
Spacegroup name	H 3 2
Unit cell lengths	99.626, 99.626, 441.539
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	47.184 - 2.900
R-factor	0.2209
Rwork	0.220
R-free	0.24420
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.011
RMSD bond angle	1.385
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.7_650))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.950
High resolution limit [Å]	2.900	2.900
Number of reflections	18934
Completeness [%]	98.3	98.8
Redundancy	4.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	277	1 mM GdCl3, 20% PEG 3350, 100 mM MgCl2, 2M NaCl and 100 mM imidazole, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K