4DVZ
Crystal structure of the Helicobacter pylori CagA oncoprotein
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE AR-NE3A |
| Synchrotron site | Photon Factory |
| Beamline | AR-NE3A |
| Temperature [K] | 95 |
| Detector technology | CCD |
| Collection date | 2011-03-06 |
| Detector | ADSC QUANTUM 270 |
| Wavelength(s) | 0.98057, 0.98086 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 95.671, 95.671, 167.924 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 74.300 - 3.190 |
| R-factor | 0.2253 |
| Rwork | 0.222 |
| R-free | 0.27860 |
| Structure solution method | MAD |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.210 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | SHARP |
| Refinement software | BUSTER (2.10.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 74.300 | 3.370 |
| High resolution limit [Å] | 3.190 | 3.190 |
| Rmerge | 0.059 | 0.468 |
| Number of reflections | 15242 | |
| <I/σ(I)> | 33.39 | 6 |
| Completeness [%] | 99.3 | 100 |
| Redundancy | 10.6 | 11.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 293 | 7% EtOH, 50mM Tris-HCl, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
