4DV6
Crystal structure of the Thermus thermophilus 30S ribosomal subunit with a 16S rRNA mutation, A915G
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-C |
Synchrotron site | APS |
Beamline | 24-ID-C |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-11-07 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.98 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 401.251, 401.251, 176.043 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 34.550 - 3.297 |
R-factor | 0.1654 |
Rwork | 0.163 |
R-free | 0.20760 |
Structure solution method | FOURIER SYNTHESIS |
RMSD bond length | 0.006 |
RMSD bond angle | 0.979 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | PHENIX (dev_978) |
Refinement software | PHENIX (dev_978) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 35.000 | 35.000 | 3.420 |
High resolution limit [Å] | 3.297 | 7.090 | 3.297 |
Rmerge | 0.097 | 0.050 | 0.731 |
Number of reflections | 210600 | ||
<I/σ(I)> | 13.5 | ||
Completeness [%] | 98.9 | 95 | 99.3 |
Redundancy | 3.8 | 3.6 | 3.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | HANGING DROP | 6.5 | 277 | MPD, pH 6.5, HANGING DROP, temperature 277K |