4DUZ
Crystal structure of the Thermus thermophilus 30S ribosomal subunit with a 16S rRNA mutation, U13C, bound with streptomycin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-11-07 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.98 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 402.524, 402.524, 173.978 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 34.561 - 3.651 |
| R-factor | 0.1587 |
| Rwork | 0.156 |
| R-free | 0.21600 |
| Structure solution method | FOURIER SYNTHESIS |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.698 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHENIX (dev_978) |
| Refinement software | PHENIX (dev_978) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 35.000 | 35.000 | 3.780 |
| High resolution limit [Å] | 3.650 | 7.840 | 3.650 |
| Rmerge | 0.080 | 0.042 | 0.519 |
| Number of reflections | 150484 | ||
| <I/σ(I)> | 13.7 | ||
| Completeness [%] | 95.8 | 89.7 | 97.6 |
| Redundancy | 3 | 3.1 | 2.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | HANGING DROP | 6.5 | 277 | MPD, pH 6.5, HANGING DROP, temperature 277K |
