4DUW
E. coli (lacZ) beta-galactosidase (G974A) in complex with allolactose
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CLSI BEAMLINE 08B1-1 |
| Synchrotron site | CLSI |
| Beamline | 08B1-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-08-01 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.11589 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 151.826, 162.635, 203.692 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 24.810 - 2.200 |
| R-factor | 0.16047 |
| Rwork | 0.160 |
| R-free | 0.21266 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 3t0d |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.081 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | CNS |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 24.810 | 2.320 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.077 | 0.283 |
| Number of reflections | 235634 | |
| <I/σ(I)> | 10 | 2.3 |
| Completeness [%] | 92.6 | 66.6 |
| Redundancy | 3.9 | 1.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 288 | 10% PEG 8000, 100 MM BIS-TRIS, 200 MM MGCL2, 100 MM NACL, 10 MM DTT , pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 288K |
