4DUU
The X-ray Crystal Structure of Full-Length type I Human Plasminogen
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-08-03 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.953697 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 110.120, 110.120, 234.815 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 27.970 - 5.200 |
| R-factor | 0.2703 |
| Rwork | 0.268 |
| R-free | 0.31170 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4dur |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.820 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Refinement software | BUSTER (2.10.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 99.700 | 5.480 |
| High resolution limit [Å] | 5.200 | 5.200 |
| Rmerge | 0.174 | 0.803 |
| Number of reflections | 5316 | |
| <I/σ(I)> | 6.8 | 2.4 |
| Completeness [%] | 91.3 | 92.8 |
| Redundancy | 5.4 | 5.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.4 | 293 | 2M sodium formate, pH 7.4, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
