4DT5
Crystal Structure of Rhagium inquisitor Antifreeze Protein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X25 |
| Synchrotron site | NSLS |
| Beamline | X25 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2011-12-16 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 1.1000 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 46.460, 46.460, 193.210 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 40.270 - 1.150 |
| R-factor | 0.13774 |
| Rwork | 0.137 |
| R-free | 0.16086 |
| Structure solution method | AB INITIO PHASING |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.502 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 27.790 | 1.170 | |
| High resolution limit [Å] | 1.150 | 5.110 | 1.140 |
| Rmerge | 0.062 | 0.049 | 0.192 |
| Number of reflections | 234706 | ||
| <I/σ(I)> | 8.4 | 13.84 | 1.36 |
| Completeness [%] | 70.2 | 98.7 | 7.4 |
| Redundancy | 2.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | EVAPORATION | 4 | 298 | 24% PEG 3350, 0.2 M (NH4)2SO4, pH 4.0, EVAPORATION, temperature 298K |
