4DT3

Crystal structure of zinc-charged lysozyme

Experimental procedure

Experimental method	SAD
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-17A
Synchrotron site	Photon Factory
Beamline	BL-17A
Temperature [K]	100
Detector technology	CCD
Collection date	2010-10-19
Detector	ADSC QUANTUM 270
Wavelength(s)	1.28286
Spacegroup name	P 43 21 2
Unit cell lengths	79.102, 79.102, 36.816
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	33.380 - 1.800
R-factor	0.183
Rwork	0.183
R-free	0.20800
Structure solution method	SAD
RMSD bond length	0.005
RMSD bond angle	1.300
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	SHELXS
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.850
High resolution limit [Å]	1.800	1.790
Number of reflections	11466
Completeness [%]	99.9	99.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	microbath	4	295	0.1M citric acid, 1M NaCl, pH 4.0, microbath, temperature 295K