4DT0
The structure of the peripheral stalk subunit E from Pyrococcus horikoshii
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | NSRRC BEAMLINE BL13B1 |
| Synchrotron site | NSRRC |
| Beamline | BL13B1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-06-24 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.979, 0.978, 0.963 |
| Spacegroup name | I 41 2 2 |
| Unit cell lengths | 111.828, 111.828, 95.466 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 26.850 - 3.650 |
| R-factor | 0.32593 |
| Rwork | 0.323 |
| R-free | 0.35669 |
| Structure solution method | MAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.064 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.5.0072) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 3.780 |
| High resolution limit [Å] | 3.650 | 3.650 |
| Rmerge | 0.060 | 0.380 |
| Number of reflections | 3564 | |
| <I/σ(I)> | 13.5 | 2.08 |
| Completeness [%] | 99.0 | 99.7 |
| Redundancy | 4.1 | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6 | 298 | 10% 2-propanol, 100mM 2-morpholinoethanesulfonic acid, sodium salt (pH 6.0), 200mM calcium acetate, 0.01 M potassium sodium tartrate tetrahydrate, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
