4DS8
Complex structure of abscisic acid receptor PYL3-(+)-ABA-HAB1 in the presence of Mn2+
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL41XU |
Synchrotron site | SPring-8 |
Beamline | BL41XU |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-04-28 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 39.745, 69.723, 82.887 |
Unit cell angles | 90.00, 101.63, 90.00 |
Refinement procedure
Resolution | 50.000 - 2.210 |
R-factor | 0.18815 |
Rwork | 0.186 |
R-free | 0.22134 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3klx |
RMSD bond length | 0.008 |
RMSD bond angle | 1.141 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.250 |
High resolution limit [Å] | 2.210 | 2.210 |
Rmerge | 0.096 | 0.330 |
Number of reflections | 20350 | |
Completeness [%] | 91.3 | 4.3 |
Redundancy | 4.8 | 3.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.8 | 291 | 28% PEG3350, 0.2M MgCl2, 0.1M Tris-HCl, pH 7.8, VAPOR DIFFUSION, HANGING DROP, temperature 291K |