4DS2
Ubiquitin conjugating enzyme (putative) from Trypanosoma cruzi
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.2.2 |
Synchrotron site | ALS |
Beamline | 8.2.2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2005-03-11 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1.0 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 60.139, 70.376, 99.117 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 40.000 - 2.630 |
R-factor | 0.2314 |
Rwork | 0.229 |
R-free | 0.27320 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.014 |
RMSD bond angle | 1.644 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | BALBES |
Refinement software | REFMAC |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 2.720 |
High resolution limit [Å] | 2.630 | 5.660 | 2.630 |
Rmerge | 0.200 | 0.211 | 0.394 |
Number of reflections | 11741 | ||
<I/σ(I)> | 6.3 | ||
Completeness [%] | 89.7 | 99.8 | 55.1 |
Redundancy | 4.1 | 4.3 | 2.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 4 | 277 | Crystallization buffer: 100 mM KH2PO4, 50 mM Sodium Citrate pH 4.0, 4% PEG 20000, 2% PEG 400. Protein buffer: 20 mM HEPES pH 7.5, 500 mM NaCl, 2 mM DTT, 5% glycerol. Cryo: ethylene glycol, vapor diffusion, temperature 277K |