4DS1
The Structure of a Yeast Dyn2-Nup159 Complex and the Molecular Basis for the Dynein Light Chain - Nuclear Pore Interaction
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-01-25 |
| Detector | RIGAKU SATURN 944+ |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 33.869, 47.971, 110.703 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.249 - 1.851 |
| R-factor | 0.1548 |
| Rwork | 0.151 |
| R-free | 0.18690 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2PG1 lacking bound peptide |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.020 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((phenix.refine: 1.7.3_928)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.200 | 1.920 |
| High resolution limit [Å] | 1.850 | 1.850 |
| Rmerge | 0.045 | |
| Number of reflections | 15081 | |
| <I/σ(I)> | 24.8 | 5.9 |
| Completeness [%] | 93.9 | 62.7 |
| Redundancy | 5.4 | 2.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 293 | 0.3 M Ammonium acetate, 5% methyl pentanediol (v/v), 35% PEG 4000 (w/v), pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
