4DS0
Cell attachment protein VP8* of a human rotavirus specifically interacts with A-type histo-blood group antigen
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-E SUPERBRIGHT |
| Detector technology | IMAGE PLATE |
| Collection date | 2010-11-24 |
| Detector | RIGAKU RAXIS HTC |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 43.750, 35.540, 44.060 |
| Unit cell angles | 90.00, 94.85, 90.00 |
Refinement procedure
| Resolution | 27.600 - 1.560 |
| R-factor | 0.1577 |
| Rwork | 0.156 |
| R-free | 0.18730 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1kqr |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.074 |
| Refinement software | PHENIX ((phenix.refine: 1.4_162)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 27.600 | 1.640 |
| High resolution limit [Å] | 1.560 | 1.560 |
| Number of reflections | 19647 | |
| Completeness [%] | 92.6 | 86.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.5 | 293 | 30% PEG 1500 Sodium acetate trihydrate, pH 4.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
