4DRY
The crystal structure of 3-oxoacyl-[acyl-carrier-protein] reductase from Rhizobium meliloti
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X25 |
| Synchrotron site | NSLS |
| Beamline | X25 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-02-15 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9787 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 86.531, 98.128, 117.220 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 49.064 - 2.500 |
| R-factor | 0.2091 |
| Rwork | 0.206 |
| R-free | 0.26370 |
| Structure solution method | SAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.050 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SOLVE |
| Refinement software | PHENIX ((phenix.refine)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.590 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.185 | 0.374 |
| Number of reflections | 35258 | |
| <I/σ(I)> | 15.1 | 8.6 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 12.6 | 12.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | 1.6 M Ammonium Sulfate, 0.1 M MES monohydrate pH 6.5 10% v/v 1,4-Dixane, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
