4DRX
GTP-Tubulin in complex with a DARPIN
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-04-11 |
| Detector | ADSC QUANTUM 315r |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 104.884, 90.596, 117.575 |
| Unit cell angles | 90.00, 90.02, 90.00 |
Refinement procedure
| Resolution | 39.190 - 2.220 |
| R-factor | 0.161 |
| Rwork | 0.159 |
| R-free | 0.19400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3ryc |
| RMSD bond length | 0.010 |
| RMSD bond angle | 0.804 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.7.2_869)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.190 | 2.350 |
| High resolution limit [Å] | 2.220 | 2.220 |
| Rmerge | 0.015 | |
| Number of reflections | 104776 | |
| <I/σ(I)> | 12.38 | 2.08 |
| Completeness [%] | 96.0 | 95 |
| Redundancy | 2.98 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7 | 292 | PEG 6K, 0.05M KCl, 0.01 M MGCL2, 0.2M LiCl, 0.01 M HEPES pH 7, VAPOR DIFFUSION, HANGING DROP, temperature 292K |
