4DRW
Crystal Structure of the Ternary Complex between S100A10, an Annexin A2 N-terminal Peptide and an AHNAK Peptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X25 |
| Synchrotron site | NSLS |
| Beamline | X25 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-10-06 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.1000 |
| Spacegroup name | P 1 |
| Unit cell lengths | 47.755, 47.791, 62.817 |
| Unit cell angles | 71.62, 71.63, 68.62 |
Refinement procedure
| Resolution | 43.340 - 3.500 |
| R-factor | 0.322 |
| Rwork | 0.296 |
| R-free | 0.32700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1bt6 |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.165 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.340 | 3.320 |
| High resolution limit [Å] | 3.260 | 3.260 |
| Number of reflections | 7306 | |
| Completeness [%] | 98.6 | 100 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 100mM sodium chloride, 200mM magnesium chloride, 50mM sodium cacodylate, 20% PEG 1000, 0.15mM CYMAL-7, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
