4DRR
Cell attachment protein VP8* of a human rotavirus specifically interacts with A-type histo-blood group antigen
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-E SUPERBRIGHT |
| Detector technology | IMAGE PLATE |
| Collection date | 2010-10-07 |
| Detector | RIGAKU RAXIS HTC |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 43.758, 34.923, 44.096 |
| Unit cell angles | 90.00, 95.46, 90.00 |
Refinement procedure
| Resolution | 23.793 - 1.500 |
| R-factor | 0.1804 |
| Rwork | 0.178 |
| R-free | 0.21600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1kqr |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.057 |
| Refinement software | PHENIX ((phenix.refine: dev_516)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 23.800 | 1.550 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Number of reflections | 20539 | |
| Completeness [%] | 95.6 | 75.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.5 | 293 | 30% PEG 1500 Sodium acetate trihydrate, pH 4.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
