4DRF
Crystal Structure of Bacterial Pnkp-C/Hen1-N Heterodimer
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-F |
| Synchrotron site | APS |
| Beamline | 21-ID-F |
| Wavelength(s) | 0.9787 |
| Spacegroup name | P 1 |
| Unit cell lengths | 59.667, 59.707, 101.542 |
| Unit cell angles | 81.27, 87.67, 88.39 |
Refinement procedure
| Resolution | 42.404 - 2.600 |
| R-factor | 0.1874 |
| Rwork | 0.184 |
| R-free | 0.24460 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.284 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 2.600 |
| Number of reflections | 41067 |
| Completeness [%] | 100.0 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.75 | 303 | 16% PEG6000, 0.5M NaCl, 25mM CaCl2, 5% methanol, 50mM HEPES, 50 mM Tris-HCl, pH 7.75, VAPOR DIFFUSION, HANGING DROP, temperature 303K |
