4DR8
Crystal structure of a peptide deformylase from Synechococcus elongatus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL7-1 |
| Synchrotron site | SSRL |
| Beamline | BL7-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-01-31 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.127092 |
| Spacegroup name | P 1 |
| Unit cell lengths | 43.400, 65.360, 69.030 |
| Unit cell angles | 80.01, 76.38, 81.71 |
Refinement procedure
| Resolution | 50.000 - 1.550 |
| R-factor | 0.164 |
| Rwork | 0.162 |
| R-free | 0.19400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | pdb deposition 1lry |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.720 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.590 |
| High resolution limit [Å] | 1.550 | 1.550 |
| Rmerge | 0.060 | 0.502 |
| Number of reflections | 99872 | |
| <I/σ(I)> | 16.2 | 3.2 |
| Completeness [%] | 95.3 | 93 |
| Redundancy | 3.9 | 3.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 290 | HAMPTON RESEARCH INDEX H12: 30% PEG 2000 MME, 150MM KBR, PH 7.5, VAPOR DIFFUSION, SITTING DROP, TEMPERATURE 290K |
