4DR1

Crystal structure of the apo 30S ribosomal subunit from Thermus thermophilus (HB8)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	CCD
Collection date	2010-11-07
Detector	ADSC QUANTUM 315
Wavelength(s)	0.98
Spacegroup name	P 41 21 2
Unit cell lengths	403.038, 403.038, 174.361
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	34.644 - 3.600
R-factor	0.1594
Rwork	0.157
R-free	0.20660
Structure solution method	FOURIER SYNTHESIS
RMSD bond length	0.012
RMSD bond angle	1.495
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHENIX (dev_978)
Refinement software	PHENIX (dev_978)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	35.000	35.000	3.730
High resolution limit [Å]	3.600	7.730	3.600
Rmerge	0.085	0.038	0.923
Number of reflections	163468
<I/σ(I)>	12
Completeness [%]	99.2	97.2	99.2
Redundancy	4.8	4.6	4.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	HANGING DROP	6.5	277	MPD, pH 6.5, HANGING DROP, temperature 277K