4DQJ
Structural Investigation of Bacteriophage Phi6 Lysin (in complex with chitotetraose)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 100 |
| Collection date | 2011-01-01 |
| Wavelength(s) | 1.075 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 43.816, 50.936, 65.020 |
| Unit cell angles | 90.00, 103.48, 90.00 |
Refinement procedure
| Resolution | 32.040 - 1.230 |
| R-factor | 0.152 |
| Rwork | 0.150 |
| R-free | 0.17000 |
| Structure solution method | SAD |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.800 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 1.250 |
| High resolution limit [Å] | 1.230 | 3.340 | 1.230 |
| Rmerge | 0.060 | 0.035 | 0.998 |
| Number of reflections | 77799 | ||
| <I/σ(I)> | 10.8 | ||
| Completeness [%] | 95.5 | 92.8 | 83.5 |
| Redundancy | 3.4 | 3.3 | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.9 | 289 | 16% PEG 3350, 0.2 M potassium nitrate, pH 6.9, VAPOR DIFFUSION, HANGING DROP, temperature 289K |
