4DPO
Crystal structure of a conserved protein MM_1583 from Methanosarcina mazei Go1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-02-12 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9790 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 59.761, 60.255, 104.312 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 39.430 - 2.730 |
| R-factor | 0.23826 |
| Rwork | 0.236 |
| R-free | 0.28291 |
| Structure solution method | SAD |
| Starting model (for MR) | None |
| RMSD bond length | 0.022 |
| RMSD bond angle | 1.962 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | AutoSol |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.810 |
| High resolution limit [Å] | 2.710 | 2.710 |
| Rmerge | 0.060 | 0.600 |
| Number of reflections | 10299 | |
| <I/σ(I)> | 13.8 | 5 |
| Completeness [%] | 98.7 | 100 |
| Redundancy | 13.7 | 14.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 293 | 0.1M Tris, pH 8.5, 2M Ammonium Sulfate, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
