4DOF
Structures of Vaccinia Virus Uracil-DNA Glycosylase in New Crystal Forms
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2011-07-03 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 92.200, 102.880, 117.240 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.850 - 2.800 |
| R-factor | 0.22364 |
| Rwork | 0.221 |
| R-free | 0.26939 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2owq |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.028 |
| Data reduction software | d*TREK |
| Data scaling software | d*TREK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.850 | 2.900 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.125 | 0.381 |
| Number of reflections | 27886 | |
| <I/σ(I)> | 7.1 | 3.2 |
| Completeness [%] | 99.5 | 99.9 |
| Redundancy | 3.4 | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 277 | 8% PEG 8000, 0.1M Tris, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
