4DNU

Crystal structure of the W285A mutant of UVB-resistance protein UVR8

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	CCD
Collection date	2011-09-10
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.97915
Spacegroup name	I 2 2 2
Unit cell lengths	66.159, 107.981, 111.846
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	32.480 - 1.764
R-factor	0.1529
Rwork	0.152
R-free	0.16850
Structure solution method	SAD
RMSD bond length	0.013
RMSD bond angle	1.226
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	SHELXS
Refinement software	PHENIX ((phenix.refine: 1.7.1_743))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.820
High resolution limit [Å]	1.760	1.760
Number of reflections	75086
Completeness [%]	97.9	90.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	291	23%(w/v) PEG 3350, 100mM Bis-Tris pH6.0, 3%(w/v) 1,5-diaminopentane dihydrochloride, VAPOR DIFFUSION, HANGING DROP, temperature 291K