4DNL

Crystal structure of a C2 domain of a protein kinase C alpha (PRKCA) from Homo sapiens at 1.90 A resolution

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.2
Synchrotron site	ALS
Beamline	8.2.2
Temperature [K]	100
Detector technology	CCD
Collection date	2011-10-15
Detector	ADSC QUANTUM 315
Wavelength(s)	0.9464,0.9795
Spacegroup name	P 32 2 1
Unit cell lengths	58.035, 58.035, 88.714
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	29.571 - 1.900
R-factor	0.1883
Rwork	0.186
R-free	0.23030
Structure solution method	MAD
RMSD bond length	0.012
RMSD bond angle	1.566
Data reduction software	XDS
Data scaling software	XSCALE (December 6, 2010)
Phasing software	SHELX
Refinement software	REFMAC (5.6.0117)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	29.571		1.970
High resolution limit [Å]	1.900	4.080	1.900
Rmerge	0.047	0.025	0.886
Number of reflections	14172	2673	2584
<I/σ(I)>	14.5	40.6	1.5
Completeness [%]	99.4	99.3	95.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5	277	30.00% polyethylene glycol 6000, 0.1M sodium citrate pH 5.0, NANODROP, VAPOR DIFFUSION, SITTING DROP, temperature 277K