4DNB
METHYLATION OF THE ECORI RECOGNITION SITE DOES NOT ALTER DNA CONFORMATION. THE CRYSTAL STRUCTURE OF D(CGCGAM6ATTCGCG) AT 2.0 ANGSTROMS RESOLUTION
Experimental procedure
| Temperature [K] | 288 |
| Detector technology | DIFFRACTOMETER |
| Detector | NICOLET |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 25.620, 40.390, 67.320 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | ? - 2.000 |
| R-factor | 0.169 |
| Refinement software | NUCLSQ |
Data quality characteristics
| Overall | |
| High resolution limit [Å] | 2.000 |
| Number of reflections | 2379 |
| Completeness [%] | 55.0 * |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 7 * | VAPOR DIFFUSION |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | WATER | ||
| 2 | 1 | 1 | MPD | ||
| 3 | 1 | 1 | MGCL2 | ||
| 4 | 1 | 1 | NA CACODYLATE | ||
| 5 | 1 | 1 | SPERMINE | ||
| 6 | 1 | 2 | WATER | ||
| 7 | 1 | 2 | MPD |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | d(CGCGAm6ATTCGCG) | 1.2 (mM) | single strand |
| 2 | 1 | drop | 1.5 (mM) | ||
| 3 | 1 | drop | sodium cacodylate | 20 (mM) | |
| 4 | 1 | drop | spermine tetrachloride | 3 (mM) | |
| 5 | 1 | drop | MPD | 10 (%) |
