4DN6

Crystal structure of a putative pilus assembly protein (cpaE) from Burkholderia thailandensis E264 at 2.80 A resolution

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL11-1
Synchrotron site	SSRL
Beamline	BL11-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2011-12-08
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97895,0.91837
Spacegroup name	P 42 21 2
Unit cell lengths	116.114, 116.114, 45.830
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.570 - 2.800
R-factor	0.2143
Rwork	0.213
R-free	0.23890
Structure solution method	MAD
RMSD bond length	0.010
RMSD bond angle	1.140
Data reduction software	XDS
Data scaling software	XSCALE (December 6, 2010)
Phasing software	SHELX
Refinement software	BUSTER-TNT (2.10.0)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	29.570	29.570	2.900
High resolution limit [Å]	2.800	5.980	2.800
Rmerge	0.057	0.025	0.795
Number of reflections	8094	1506	1484
<I/σ(I)>	15.71	38.5	1.58
Completeness [%]	98.6	98	98.5
Redundancy	6.89

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	277	10.0% PEG-6000, 0.1M HEPES pH 7.0, NANODROP, VAPOR DIFFUSION, SITTING DROP, temperature 277K