4DN6
Crystal structure of a putative pilus assembly protein (cpaE) from Burkholderia thailandensis E264 at 2.80 A resolution
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL11-1 |
Synchrotron site | SSRL |
Beamline | BL11-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2011-12-08 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.97895,0.91837 |
Spacegroup name | P 42 21 2 |
Unit cell lengths | 116.114, 116.114, 45.830 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 29.570 - 2.800 |
R-factor | 0.2143 |
Rwork | 0.213 |
R-free | 0.23890 |
Structure solution method | MAD |
RMSD bond length | 0.010 |
RMSD bond angle | 1.140 |
Data reduction software | XDS |
Data scaling software | XSCALE (December 6, 2010) |
Phasing software | SHELX |
Refinement software | BUSTER-TNT (2.10.0) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 29.570 | 29.570 | 2.900 |
High resolution limit [Å] | 2.800 | 5.980 | 2.800 |
Rmerge | 0.057 | 0.025 | 0.795 |
Number of reflections | 8094 | 1506 | 1484 |
<I/σ(I)> | 15.71 | 38.5 | 1.58 |
Completeness [%] | 98.6 | 98 | 98.5 |
Redundancy | 6.89 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7 | 277 | 10.0% PEG-6000, 0.1M HEPES pH 7.0, NANODROP, VAPOR DIFFUSION, SITTING DROP, temperature 277K |