4DML
3-oxoacyl-[acyl-carrier-protein] reductase from Synechococcus elongatus PCC 7942
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | PAL/PLS BEAMLINE 6C1 |
| Synchrotron site | PAL/PLS |
| Beamline | 6C1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-12-14 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9795, 0.9796, 0.9718 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 63.911, 117.932, 125.329 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 43.340 - 2.500 |
| R-factor | 0.20035 |
| Rwork | 0.197 |
| R-free | 0.26201 |
| Structure solution method | MAD |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.749 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.340 | |
| High resolution limit [Å] | 2.500 | 2.500 |
| Number of reflections | 31758 | |
| Completeness [%] | 94.8 | 94.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 291 | 21% PEG3350, 0.1M HEPES, 0.16M Lithium sulfate monohydrate, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
