4DMF
Crystal structure of the CFTR inhibitory factor Cif with the H177A mutation
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X6A |
| Synchrotron site | NSLS |
| Beamline | X6A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-10-11 |
| Detector | ADSC QUANTUM 270 |
| Wavelength(s) | 0.9770 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 167.766, 83.784, 89.045 |
| Unit cell angles | 90.00, 100.39, 90.00 |
Refinement procedure
| Resolution | 45.981 - 2.120 |
| R-factor | 0.1683 |
| Rwork | 0.166 |
| R-free | 0.20580 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | CHAIN A OF PDB ENTRY 3KD2 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.994 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((phenix.refine: 1.6.1_357)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.981 | 2.200 |
| High resolution limit [Å] | 2.120 | 2.120 |
| Number of reflections | 68803 | |
| <I/σ(I)> | 12.7 | 4 |
| Completeness [%] | 99.8 | 99.7 |
| Redundancy | 4.2 | 4.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5 | 291 | 16% PEG 8000, 0.125M calcium chloride, 0.1M sodium acetate, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
