4DME
GCN4 leucine zipper domain in a trimeric oligomerization state
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-E SUPERBRIGHT |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-09-02 |
| Detector | RIGAKU SATURN 944 |
| Wavelength(s) | 1.5418 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 61.179, 34.379, 78.117 |
| Unit cell angles | 90.00, 139.68, 90.00 |
Refinement procedure
| Resolution | 50.540 - 2.200 |
| R-factor | 0.22856 |
| Rwork | 0.226 |
| R-free | 0.28733 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ij2 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.387 |
| Data reduction software | d*TREK |
| Data scaling software | d*TREK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.540 |
| High resolution limit [Å] | 2.200 |
| Rmerge | 0.094 |
| Number of reflections | 5443 |
| <I/σ(I)> | 9.7 |
| Completeness [%] | 99.5 |
| Redundancy | 2.71 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 298 | 0.2 M Ammonium Sulfate, 0.1 M MES buffer, 30% (w/v) PEG MME 5000, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
