4DMB
X-ray structure of human hepatitus C virus NS5A-transactivated protein 2 at the resolution 1.9A, Northeast Structural Genomics Consortium (NESG) Target HR6723
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X4C |
| Synchrotron site | NSLS |
| Beamline | X4C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-02-01 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 0.97906 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 68.393, 68.393, 174.452 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 42.295 - 1.900 |
| R-factor | 0.1768 |
| Rwork | 0.175 |
| R-free | 0.20680 |
| Structure solution method | SAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.988 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | AutoSol |
| Refinement software | PHENIX (dev_988) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.970 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.087 | 0.455 |
| Number of reflections | 59948 | |
| <I/σ(I)> | 18.2 | 1.8 |
| Completeness [%] | 96.1 | 71.4 |
| Redundancy | 4.7 | 2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6 | 293 | Protein solution: 100mM NaCl, 5mM DTT, 0.02% NaN3, 10mM Tris-HCl (pH 7.5) . Reservoir solution:Mg(NO3)2 0.1M, MES 0.1M, PEG4000 40% (w/v), microbatch under oil, temperature 293KK |
