4DM5
Putative Osmotically inducible lipoprotein OsmE characterization by Xray crystallography
Experimental procedure
| Experimental method | SAD |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2011-11-16 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 0.97926 |
| Spacegroup name | P 1 |
| Unit cell lengths | 39.130, 44.260, 59.280 |
| Unit cell angles | 94.20, 93.92, 116.07 |
Refinement procedure
| Resolution | 39.490 - 1.500 |
| R-factor | 0.18002 |
| Rwork | 0.179 |
| R-free | 0.20854 |
| Structure solution method | SAD |
| RMSD bond length | 0.023 |
| RMSD bond angle | 2.467 |
| Data scaling software | XDS |
| Phasing software | SHELXS |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.490 | 1.540 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Number of reflections | 104647 | |
| Completeness [%] | 92.2 | 92.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 9 | 298 | 0.01M Na-Borate, 1.7M Na-Citrate, Glycerol 2.5%, 0.01M Tris, 0.125M NaCl, 0.001M DTT , pH 9, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
