4DM4
The conserved domain of yeast Cdc73
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2009-01-01 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.5 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 90.938, 96.893, 114.029 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.190 |
| R-factor | 0.22275 |
| Rwork | 0.221 |
| R-free | 0.26505 |
| Structure solution method | SAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.061 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.5.0072) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 73.840 | 2.240 |
| High resolution limit [Å] | 2.190 | 2.200 |
| Number of reflections | 26095 | |
| Completeness [%] | 100.0 | 100 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 287 | 2.7M Sodium formate, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 287K |
