4DLB
Structure of S-nitrosoglutathione reductase from tomato (Solanum lycopersicum) crystallized in presence of NADH and glutathione
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2011-10-14 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 0.98011 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 76.640, 78.040, 124.260 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 28.790 - 2.100 |
| R-factor | 0.1841 |
| Rwork | 0.181 |
| R-free | 0.24280 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1mc5 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.140 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | MOLREP |
| Refinement software | BUSTER (2.10.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 28.800 | 2.220 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.124 | 0.757 |
| Number of reflections | 44113 | |
| <I/σ(I)> | 9.21 | 1.83 |
| Completeness [%] | 99.3 | 97.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 0.1 M MES pH 6.5, 13% PEG 20000, 4% glycerol (plus 2.5 mM NADH and 5 mM glutathione in the drop), VAPOR DIFFUSION, HANGING DROP, temperature 293K |
