4DKY

Crystal structure Analysis of N terminal region containing the dimerization domain and DNA binding domain of HU protein(Histone like protein-DNA binding) from Mycobacterium tuberculosis [H37Ra]

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X29A
Synchrotron site	NSLS
Beamline	X29A
Temperature [K]	100
Detector technology	CCD
Collection date	2010-10-29
Detector	ADSC QUANTUM 315
Wavelength(s)	0.979
Spacegroup name	P 1 2 1
Unit cell lengths	55.115, 37.052, 64.854
Unit cell angles	90.00, 115.29, 90.00

Refinement procedure

Resolution	31.920 - 2.478
R-factor	0.18596
Rwork	0.183
R-free	0.24786
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3C4I
RMSD bond length	0.026
RMSD bond angle	2.390
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHASER (2.3.0)
Refinement software	REFMAC

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.542
High resolution limit [Å]	2.478	6.780	2.478
Rmerge	0.094	0.043	0.575
Number of reflections	8546
<I/σ(I)>	7.6
Completeness [%]	81.3	98.9	99
Redundancy	3.8	3.5	3.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.7	295	0.1M Tris-HCl, pH 6.7, 2.5M sodium formate, 10mM MnCl2, VAPOR DIFFUSION, HANGING DROP, temperature 295K