4DKN
Crystal structure of amphioxus green fluorescent protein, GFPA1
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.2.1 |
Synchrotron site | ALS |
Beamline | 8.2.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2008-02-29 |
Detector | ADSC QUANTUM 315 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 59.254, 125.576, 106.465 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 32.550 - 1.350 |
R-factor | 0.192 |
Rwork | 0.192 |
R-free | 0.20600 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3GJV |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | CNS (1.2) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.400 |
High resolution limit [Å] | 1.350 | 1.350 |
Number of reflections | 84939 | |
<I/σ(I)> | 3 | |
Completeness [%] | 97.4 | 83.9 |
Redundancy | 6.7 | 5.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 5.5 | 100MM CITRIC ACID, 30% PEG 4000, 3% ISOPROPANOL, PH 5.5, VAPOR DIFFUSION, HANGING DROP, TEMPERATURE 298K |