4DKM
Crystal Structure of Amphioxus GFPc1a
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.1 |
| Synchrotron site | ALS |
| Beamline | 8.2.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-02-29 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 158.760, 130.460, 106.330 |
| Unit cell angles | 90.00, 128.39, 90.00 |
Refinement procedure
| Resolution | 45.020 - 1.950 |
| Rwork | 0.293 |
| R-free | 0.32040 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2g3o |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.020 |
| High resolution limit [Å] | 1.950 | 1.950 |
| Number of reflections | 122637 | |
| Completeness [%] | 99.7 | 98.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 298 | 28% PEG 8000, 1M NACL, 100MM HEPES, , pH 7, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
