4DK0
Crystal structure of MacA from Actinobacillus actinomycetemcomitans
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | PAL/PLS BEAMLINE 6C1 |
| Synchrotron site | PAL/PLS |
| Beamline | 6C1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-01-15 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 1.0000, 0.9794, 0.9796, 1.1271 |
| Spacegroup name | P 6 2 2 |
| Unit cell lengths | 109.188, 109.188, 255.442 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 9.991 - 3.500 |
| R-factor | 0.3435 |
| Rwork | 0.338 |
| R-free | 0.38990 |
| Structure solution method | MAD |
| RMSD bond length | 0.015 |
| RMSD bond angle | 2.095 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SOLVE |
| Refinement software | PHENIX ((phenix.refine: 1.7.2_869)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.110 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Number of reflections | 17810 | |
| Completeness [%] | 97.2 | 87.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | EVAPORATION | 8.5 | 277 | 0.01M Nickel chloride, 0.1M Tris-HCl, 1M Lithium Sulfate, pH 8.5, EVAPORATION, temperature 277K |
