4DJM

Crystal structure of the E. coli chaperone DraB

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-D
Synchrotron site	APS
Beamline	23-ID-D
Temperature [K]	100
Detector technology	CCD
Collection date	2009-06-20
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	0.9724
Spacegroup name	P 1
Unit cell lengths	59.450, 98.410, 100.880
Unit cell angles	84.20, 89.80, 83.80

Refinement procedure

Resolution	30.000 - 2.520
R-factor	0.20709
Rwork	0.206
R-free	0.26871
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2co6
RMSD bond length	0.023
RMSD bond angle	2.151
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	REFMAC (5.5.0109)

Data quality characteristics

	Overall
Low resolution limit [Å]	30.000
High resolution limit [Å]	2.500
Rmerge	0.109
Number of reflections	74832
<I/σ(I)>	13.4
Completeness [%]	98.4
Redundancy	3.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	293	protein solution: 15 mg/ml protein, 20 mM Tris pH 8.0, 200 mM NaCl, 10 % glycerol well solution: 200 mM MgCl2, 100 mM Bis-Tris pH 6.5, 25% PEG3350 protein and well solutions mixed 1:1, VAPOR DIFFUSION, HANGING DROP, temperature 293K