4DJG
Crystal structure of the coiled-coil 1 domain of actin-binding protein SCAB1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2009-02-10 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 48.185, 48.185, 77.919 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 18.860 - 1.900 |
| R-factor | 0.2066 |
| Rwork | 0.205 |
| R-free | 0.24400 |
| Structure solution method | SIRAS |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.154 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SHARP |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 20.000 | 20.000 | 1.930 |
| High resolution limit [Å] | 1.900 | 5.130 | 1.900 |
| Rmerge | 0.057 | 0.031 | 0.381 |
| Number of reflections | 7516 | ||
| <I/σ(I)> | 14.4 | ||
| Completeness [%] | 97.4 | 99.6 | 96.2 |
| Redundancy | 8.7 | 7.3 | 8.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.3 | 293 | 0.1M Bis-Tris PH5.3 22.5% PEG 3350, vapor diffusion, hanging drop, temperature 293K |
