4DJ7
Structure of the hemagglutinin complexed with 3SLN from a highly pathogenic H7N7 influenza virus
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-ID |
Synchrotron site | APS |
Beamline | 22-ID |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2009-08-09 |
Detector | MAR scanner 300 mm plate |
Wavelength(s) | 1.0 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 200.279, 115.723, 117.656 |
Unit cell angles | 90.00, 124.39, 90.00 |
Refinement procedure
Resolution | 50.000 - 2.810 |
R-factor | 0.20922 |
Rwork | 0.207 |
R-free | 0.24263 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.009 |
RMSD bond angle | 1.110 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.900 |
High resolution limit [Å] | 2.800 | 2.800 |
Number of reflections | 49829 | |
Completeness [%] | 97.8 | 90.9 |
Redundancy | 3.7 | 3.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | EVAPORATION | 8 | 298 | 20 % PEG 8000 and 1 M lithium chloride, Microbatch under oil, temperature 298K, EVAPORATION |