4DJ2
Unwinding the Differences of the Mammalian PERIOD Clock Proteins from Crystal Structure to Cellular Function
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2010-07-16 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 0.9807 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 100.340, 56.870, 100.950 |
| Unit cell angles | 90.00, 90.07, 90.00 |
Refinement procedure
| Resolution | 49.549 - 2.750 |
| R-factor | 0.2138 |
| Rwork | 0.208 |
| R-free | 0.28230 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.291 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | MOLREP |
| Refinement software | PHENIX ((phenix.refine: 1.6.1_357)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.800 |
| High resolution limit [Å] | 2.750 | 2.750 |
| Number of reflections | 29780 | |
| Completeness [%] | 99.0 | 99.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 100 mM Tris, 0.15 M NH4OAc, 16% PEG 3350, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
